BASIC SCIENCE RESEARCH
Tracks
STREAM 3
Wednesday, November 10, 2021 |
3:00 PM - 5:00 PM |
STREAM 3 |
Speaker
Dr Francis Makokha
Mount Kenya University
Immune receptor recombination patterns among Kenyan breast cancer patients
Abstract
The role of the immune system in breast cancer development and progression is an active area of research with potential to revolutionize the management of the disease through immunotherapeutic interventions. In Sub-Saharan Africa, there are no genomic studies on immune receptor recombination and their role in breast cancer. Our current study aimed to recover T- and B-cell receptor recombination reads from RNA-seq and whole exome sequencing (WXS) data from Kenyan patients and assess their utility in studying the infiltrating lymphocytes in the tumor microenvironment. We aligned the RNA-seq and WXS sequencing reads to human genome GRCh38 version and used human immune receptor V and J sequences from The International Immunogenetics Information System (IMGT) to screen the alignments for matching recombination reads. We found that the recombination reads for B-cells were significantly higher than alpha-beta T-cells in the tumor RNA-seq dataset (p-value = 0.0236). In contrast, more alpha-beta T-cell than B-cell recombination reads were recovered from the exome dataset (p-value = 0.0288).
Reem Salah Eldin
Breast cancer and smell: hints from epigenetic and functional alteration of the olfaction
Dr Samrin Habbani
Purdue University
Point-of-care test for cervical cancer
Dr Evelyn Jiagge
Henry Ford Health System
Precision Medicine for African Breast Cancer (PMABC): bringing African researchers together to study African breast cancers in Africa
Dr Solomon Rotimi
Covenant University
The raison d’être of African cancer genomics
Facilitators
Sulma Mohammed
Purdue University